1. Cells grown in static culture conditions had a thin, faintlyvisible extracellular matrix.
2. As in T. oligopyrenoides, a peripheral ER system was not visible.
3. The single multilobed chloroplast had a 1.5-mm eccentric pyrenoid with a moderately electron dense matrix containing numerous tubular thylakoids.
4. The pyrenoid was exposed to the cytoplasm, usually surrounded by a starch sheath.
5. Starch was also found scattered throughout the cell closely appressed to the chloroplast lobes.
6. Examination of several hundred cells revealed that each cell has a single chloroplast possessing three to five lobes projecting from the pyrenoid. The lobes extended to and occupied most of the cell periphery. Phycobilisomes were clearly seen, and a peripheral encircling thylakoid was absent.
7. The irregularly spherical 1.5-mm nucleus was eccentric, lying in close proximity to the pyrenoid . A single nucleolus 0.5–0.6 mm in diameter was found in the nucleus center.
8. Heterochromatin was never seen.
9. Mitochondria, or profiles of a possible mitochondrial. Reticulum was seen in a single plane of sectioning, were found throughout the cell.
10. Mitochondrial cristae were distinctly tubular.
11. Golgi bodies were usually found at the cell periphery in areas not occupied by chloroplast lobes but occasionally were seen near the cell center. Golgi cisternae were evenly spaced, and the cis region of each Golgi body was invariably closely associated with a mitochondrion .
From Patrick J. Keeling ,at Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada and Canadian Institute for Advanced Research, Integrated Microbial Biodiversity program, Canada.
Keeling PJ, Burki F, Wilcox HM, Allam B, Allen EE, Amaral-Zettler LA, et al. (2014) The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing. PLoS Biol 12 (6): e1001889.